quantinova reverse transcription kit (Qiagen)
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Quantinova Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1089 article reviews
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Reverse Transcription:Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival Article Snippet: .. Total RNA from MSCs was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany, catalog# 74134,), and the reverse transcription was performed with Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival. Article Snippet: .. Total RNA from MSCs was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany, catalog# 74134,), and the reverse transcription was performed with Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the Article Title: Epigenetic and microRNA-mediated regulation of pulmonary fibrosis and immune dysregulation in fatal COVID-19. Article Snippet: Total RNA was extracted from the lung samples using TRIzol (Invitrogen) following tissue disruption with the TissueLyser LT (Qiagen). .. Approximately 2 μg of RNA was treated with Turbo DNAse I (Invitrogen), followed by cDNA synthesis using the Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the Article Title: Co-culturing matrigel-encapsulated post-cryopreserved human ovarian cortical tissue with mesenchymal stem cells to improve In situ follicular activation and early development. Article Snippet: Recent advances in culturing cryopreserved ovarian cortical tissue (OCT) offer a promising strategy for fertility preservation, especially for patients undergoing gonadotoxic treatments.. This study evaluated whether Matrigel encapsulation combined with mesenchymal stem cell (MSC) co-culture enhances folliculogenesis in postcryopreserved human OCT during an 8-day in vitro culture.. Thawed human OCT fragments were encapsulated in Matrigel and cultured in the upper chamber of a transwell plate, with MSCs in the lower chamber. Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells. Article Snippet: Before qRT- PCR Array, RNA was isolated using the RNeasy Plus mini Kit (Qiagen, Milan, Italy, Cat. no. 74104) following the provided protocol. .. RNA quality and quantity were evaluated using a Nanodrop spectrophotometer (ND- 1000, Nanodrop Technologies, Wilmington, DE, USA), then reverse transcribed to cDNA using Isolation:Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the Synthesized:Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the Quantitative RT-PCR:Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the SYBR Green Assay:Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the Expressing:Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the Purification:Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the Real-time Polymerase Chain Reaction:Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the cDNA Synthesis:Article Title: Epigenetic and microRNA-mediated regulation of pulmonary fibrosis and immune dysregulation in fatal COVID-19. Article Snippet: Total RNA was extracted from the lung samples using TRIzol (Invitrogen) following tissue disruption with the TissueLyser LT (Qiagen). .. Approximately 2 μg of RNA was treated with Turbo DNAse I (Invitrogen), followed by cDNA synthesis using the Cell Culture:Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the Spectrophotometry:Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells. Article Snippet: Before qRT- PCR Array, RNA was isolated using the RNeasy Plus mini Kit (Qiagen, Milan, Italy, Cat. no. 74104) following the provided protocol. .. RNA quality and quantity were evaluated using a Nanodrop spectrophotometer (ND- 1000, Nanodrop Technologies, Wilmington, DE, USA), then reverse transcribed to cDNA using Polymerase Chain Reaction:Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells. Article Snippet: Before qRT- PCR Array, RNA was isolated using the RNeasy Plus mini Kit (Qiagen, Milan, Italy, Cat. no. 74104) following the provided protocol. .. RNA quality and quantity were evaluated using a Nanodrop spectrophotometer (ND- 1000, Nanodrop Technologies, Wilmington, DE, USA), then reverse transcribed to cDNA using |
