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quantinova reverse transcription kit  (Qiagen)


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    Structured Review

    Qiagen quantinova reverse transcription kit
    Quantinova Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantinova+reverse+transcription+kit/QuantiNova+Reverse+Transcription+Kit/pmc13129375-154-12-16
    Average 96 stars, based on 1089 article reviews
    quantinova reverse transcription kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival
    Article Snippet: .. Total RNA from MSCs was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany, catalog# 74134,), and the reverse transcription was performed with QuantiNova Reverse Transcription Kit (Qiagen, catalog# 205411) according to the manufacturer’s instructions. .. Quantitative PCR was conducted to assess target mRNA levels using TaqMan Fast Advanced Master Mix (Applied Biosystems, Waltham, MA, USA, catalog# 4444963) and Gene Expression TaqMan Assays (Applied Biosystems) targeting VEGFA (Exon 3–4) (Hs00900055_m1) and GAPDH (Hs02758991_g1) as per the manufacturer’s protocols.

    Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival.
    Article Snippet: .. Total RNA from MSCs was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany, catalog# 74134,), and the reverse transcription was performed with QuantiNova Reverse Transcription Kit (Qiagen, catalog# 205411) according to the manufacturer’s instructions. .. Quantitative PCR was conducted to assess target mRNA levels using TaqMan Fast Advanced Master Mix (Applied Biosystems, Waltham, MA, USA, catalog# 4444963) and Gene Expression TaqMan Assays (Applied Biosystems) targeting VEGFA (Exon 3–4) (Hs00900055_m1) and GAPDH (Hs02758991_g1) as per the manufacturer’s protocols.

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the QuantiNova® reverse transcription kit (Qiagen, Hilden, Germany) for quantitative real-time polymerase chain reaction (qPCR) analysis, as described by . qPCR was performed in triplicate on a LightCycler® 480 II system (Roche, Mannheim, Germany) to quantify the expression levels of target genes. ..

    Article Title: Epigenetic and microRNA-mediated regulation of pulmonary fibrosis and immune dysregulation in fatal COVID-19.
    Article Snippet: Total RNA was extracted from the lung samples using TRIzol (Invitrogen) following tissue disruption with the TissueLyser LT (Qiagen). .. Approximately 2 μg of RNA was treated with Turbo DNAse I (Invitrogen), followed by cDNA synthesis using the QuantiNova Reverse Transcription Kit (Qiagen). .. The cDNAs were subjected to RT‒qPCR using 1x SYBR Green Universal Master Mix (Applied Biosystems) on a StepOnePlus system (Applied Biosystems).

    Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling
    Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the QuantiNova Reverse Transcription Kit (205411, Qiagen). .. Quantitative real-time PCR (RT-qPCR) was performed on a Rotor-Gene Q system (Qiagen) followed by analyses using Rotor-Gene Q software (version 2.3.5).

    Article Title: Co-culturing matrigel-encapsulated post-cryopreserved human ovarian cortical tissue with mesenchymal stem cells to improve In situ follicular activation and early development.
    Article Snippet: Recent advances in culturing cryopreserved ovarian cortical tissue (OCT) offer a promising strategy for fertility preservation, especially for patients undergoing gonadotoxic treatments.. This study evaluated whether Matrigel encapsulation combined with mesenchymal stem cell (MSC) co-culture enhances folliculogenesis in postcryopreserved human OCT during an 8-day in vitro culture.. Thawed human OCT fragments were encapsulated in Matrigel and cultured in the upper chamber of a transwell plate, with MSCs in the lower chamber.

    Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells.
    Article Snippet: Before qRT- PCR Array, RNA was isolated using the RNeasy Plus mini Kit (Qiagen, Milan, Italy, Cat. no. 74104) following the provided protocol. .. RNA quality and quantity were evaluated using a Nanodrop spectrophotometer (ND- 1000, Nanodrop Technologies, Wilmington, DE, USA), then reverse transcribed to cDNA using QuantiNova Reverse Transcription Kit (Qiagen, Milan, Italy) for the Human Apoptosis PCR Array and the RT2 First Strand cDNA Kit (Qiagen, Milan, Italy) for The Human Extracellular Matrix and Adhesion Molecules Array [46, 47]. ..

    Isolation:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Synthesized:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling
    Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the QuantiNova Reverse Transcription Kit (205411, Qiagen). .. Quantitative real-time PCR (RT-qPCR) was performed on a Rotor-Gene Q system (Qiagen) followed by analyses using Rotor-Gene Q software (version 2.3.5).

    Quantitative RT-PCR:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    SYBR Green Assay:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Expressing:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the QuantiNova® reverse transcription kit (Qiagen, Hilden, Germany) for quantitative real-time polymerase chain reaction (qPCR) analysis, as described by . qPCR was performed in triplicate on a LightCycler® 480 II system (Roche, Mannheim, Germany) to quantify the expression levels of target genes. ..

    Purification:

    Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the QuantiNova® reverse transcription kit (Qiagen, Hilden, Germany) for quantitative real-time polymerase chain reaction (qPCR) analysis, as described by . qPCR was performed in triplicate on a LightCycler® 480 II system (Roche, Mannheim, Germany) to quantify the expression levels of target genes. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the QuantiNova® reverse transcription kit (Qiagen, Hilden, Germany) for quantitative real-time polymerase chain reaction (qPCR) analysis, as described by . qPCR was performed in triplicate on a LightCycler® 480 II system (Roche, Mannheim, Germany) to quantify the expression levels of target genes. ..

    cDNA Synthesis:

    Article Title: Epigenetic and microRNA-mediated regulation of pulmonary fibrosis and immune dysregulation in fatal COVID-19.
    Article Snippet: Total RNA was extracted from the lung samples using TRIzol (Invitrogen) following tissue disruption with the TissueLyser LT (Qiagen). .. Approximately 2 μg of RNA was treated with Turbo DNAse I (Invitrogen), followed by cDNA synthesis using the QuantiNova Reverse Transcription Kit (Qiagen). .. The cDNAs were subjected to RT‒qPCR using 1x SYBR Green Universal Master Mix (Applied Biosystems) on a StepOnePlus system (Applied Biosystems).

    Cell Culture:

    Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling
    Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the QuantiNova Reverse Transcription Kit (205411, Qiagen). .. Quantitative real-time PCR (RT-qPCR) was performed on a Rotor-Gene Q system (Qiagen) followed by analyses using Rotor-Gene Q software (version 2.3.5).

    Spectrophotometry:

    Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells.
    Article Snippet: Before qRT- PCR Array, RNA was isolated using the RNeasy Plus mini Kit (Qiagen, Milan, Italy, Cat. no. 74104) following the provided protocol. .. RNA quality and quantity were evaluated using a Nanodrop spectrophotometer (ND- 1000, Nanodrop Technologies, Wilmington, DE, USA), then reverse transcribed to cDNA using QuantiNova Reverse Transcription Kit (Qiagen, Milan, Italy) for the Human Apoptosis PCR Array and the RT2 First Strand cDNA Kit (Qiagen, Milan, Italy) for The Human Extracellular Matrix and Adhesion Molecules Array [46, 47]. ..

    Polymerase Chain Reaction:

    Article Title: Gene Expression Alterations Associated With Resveratrol-Induced Antiproliferative Effects and S-Phase Cell Cycle Arrest in Osteosarcoma Cancer Cells.
    Article Snippet: Before qRT- PCR Array, RNA was isolated using the RNeasy Plus mini Kit (Qiagen, Milan, Italy, Cat. no. 74104) following the provided protocol. .. RNA quality and quantity were evaluated using a Nanodrop spectrophotometer (ND- 1000, Nanodrop Technologies, Wilmington, DE, USA), then reverse transcribed to cDNA using QuantiNova Reverse Transcription Kit (Qiagen, Milan, Italy) for the Human Apoptosis PCR Array and the RT2 First Strand cDNA Kit (Qiagen, Milan, Italy) for The Human Extracellular Matrix and Adhesion Molecules Array [46, 47]. ..



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    Image Search Results


    Fortilin physically binds MEF2C. A, quantitative RT–PCR (qRT–PCR) analysis of THP1 WT-fortilin and THP1 KO-fortilin cells ( n = 3, p < 0.0001, two-sample t test). B – E, MST to characterize the interaction of fortilin with MEF2 family proteins, MEF2A, MEF2B, MEF2C, and MEF2D. K d s were calculated using NanoTemper Analysis software based on the relative F norm values. F – I, in vivo forward co-IP assays. Fortilin was immunoprecipitated from the TCLs of 293T cells using an α-fortilin Ab. Successful IP of fortilin and co-IP of MEF2C and other MEF2 isoforms were assessed by Western blotting using α-fortilin Ab and Abs against MEF2A, MEF2B, MEF2C, and MEF2D, respectively. Total protein was visualized in the SDS-gel containing TCE. J, in vivo reverse co-IP assay. MEF2C was immunoprecipitated from the 293T TCL using an α-MEF2C Ab. Successful IP of MEF2C and co-IP of fortilin were assessed by Western blotting using α-MEF2C and α-fortilin Abs, respectively. Total protein was visualized in an SDS-gel containing TCE. K and L, PLA to evaluate the fortilin–MEF2C interaction. Phorbol 12-myristate 13-acetate-treated THP1 WT-fortilin and THP1 KO-fortilin cells cultured on chamber slides were subjected to a standard PLA assay using rabbit α-fortilin and mouse α-MEF2C Abs. Nuclei were counterstained by DAPI ( blue ). Red puncta indicated that fortilin and MEF2C were located within 30 nm of each other. The scale bar represents 50 μm. K, the PLA interaction index was calculated as the number of PLA puncta per nucleus within a field of view and expressed in AU. Three fields were quantified for each sample, and three independent experiments were performed. Statistical significance was evaluated using a Welch’s two-sample t test ( L ). Ab, antibody; AU, arbitrary unit; DAPI, 4′,6-diamidino-2-phenylindole; α-FLAG, anti-FLAG (DYKDDDDK) antibody (Ab); F norlm , normalized fluorescence value; α-fortilin, rabbit anti-fortilin monoclonal Ab; Fortilin KO, THP1 cells in which the fortilin ( TPT1 ) genes have been deleted by the CRISPR–Cas9 technology (THP1 KO-fortilin ); Fortilin WT, THP1 cells expressing WT fortilin (THP1 WT-fortilin ); α-His 6 , anti-hexahistidine Ab; IB, immunoblot; IgG, normal rabbit immunoglobulin G; INPUT, 10% of TCLs used for IP; IP, immunoprecipitation; α-MEF2C, anti-MEF2C Ab; MST, microscale thermophoresis; PLA, proximity ligation assay; TCE, 2,2,2-trichloroethanol; TCL, total cell lysate.

    Journal: The Journal of Biological Chemistry

    Article Title: Fortilin binds and stabilizes MEF2C, activates it through phosphorylation, and drives transcription of the cell structural and survival protein CTNNA3

    doi: 10.1016/j.jbc.2026.111417

    Figure Lengend Snippet: Fortilin physically binds MEF2C. A, quantitative RT–PCR (qRT–PCR) analysis of THP1 WT-fortilin and THP1 KO-fortilin cells ( n = 3, p < 0.0001, two-sample t test). B – E, MST to characterize the interaction of fortilin with MEF2 family proteins, MEF2A, MEF2B, MEF2C, and MEF2D. K d s were calculated using NanoTemper Analysis software based on the relative F norm values. F – I, in vivo forward co-IP assays. Fortilin was immunoprecipitated from the TCLs of 293T cells using an α-fortilin Ab. Successful IP of fortilin and co-IP of MEF2C and other MEF2 isoforms were assessed by Western blotting using α-fortilin Ab and Abs against MEF2A, MEF2B, MEF2C, and MEF2D, respectively. Total protein was visualized in the SDS-gel containing TCE. J, in vivo reverse co-IP assay. MEF2C was immunoprecipitated from the 293T TCL using an α-MEF2C Ab. Successful IP of MEF2C and co-IP of fortilin were assessed by Western blotting using α-MEF2C and α-fortilin Abs, respectively. Total protein was visualized in an SDS-gel containing TCE. K and L, PLA to evaluate the fortilin–MEF2C interaction. Phorbol 12-myristate 13-acetate-treated THP1 WT-fortilin and THP1 KO-fortilin cells cultured on chamber slides were subjected to a standard PLA assay using rabbit α-fortilin and mouse α-MEF2C Abs. Nuclei were counterstained by DAPI ( blue ). Red puncta indicated that fortilin and MEF2C were located within 30 nm of each other. The scale bar represents 50 μm. K, the PLA interaction index was calculated as the number of PLA puncta per nucleus within a field of view and expressed in AU. Three fields were quantified for each sample, and three independent experiments were performed. Statistical significance was evaluated using a Welch’s two-sample t test ( L ). Ab, antibody; AU, arbitrary unit; DAPI, 4′,6-diamidino-2-phenylindole; α-FLAG, anti-FLAG (DYKDDDDK) antibody (Ab); F norlm , normalized fluorescence value; α-fortilin, rabbit anti-fortilin monoclonal Ab; Fortilin KO, THP1 cells in which the fortilin ( TPT1 ) genes have been deleted by the CRISPR–Cas9 technology (THP1 KO-fortilin ); Fortilin WT, THP1 cells expressing WT fortilin (THP1 WT-fortilin ); α-His 6 , anti-hexahistidine Ab; IB, immunoblot; IgG, normal rabbit immunoglobulin G; INPUT, 10% of TCLs used for IP; IP, immunoprecipitation; α-MEF2C, anti-MEF2C Ab; MST, microscale thermophoresis; PLA, proximity ligation assay; TCE, 2,2,2-trichloroethanol; TCL, total cell lysate.

    Article Snippet: Briefly, we harvested THP1 KO-fortilin and THP1 WT-fortilin cells, isolated total RNA using the GeneJET RNA Purification Kit (Thermo Fisher Scientific, catalog no.: K0731), and subjected 50 ng of total RNA to real-time RT–qPCR using the QuantiNova Probe RT–PCR kit (QIAGEN, catalog no.: 208354) with the following primer and probe sets (Integrated DNA Technologies).

    Techniques: Quantitative RT-PCR, Software, In Vivo, Co-Immunoprecipitation Assay, Immunoprecipitation, Western Blot, SDS-Gel, Cell Culture, Fluorescence, CRISPR, Expressing, Microscale Thermophoresis, Proximity Ligation Assay